Fermentation Lab Report Essay Example for Free

Fermentation Lab Report Essay Increased production of CO2 is a result of increased temperatures acceleration of the rate of fermentation. Abstract: We have tested the affects of increased temperature above room temperature on the rate of fermentation of yeast. We had 6 flasks filled with 6mL DI water, 2mL Yeast suspension and 6mL glucose of which 3 were at 25 °C and 3 were at 37 °C. The flasks at 37 °C had each mixture pre-heated at 37 °C for 2 minutes before being combined and then added to the flask where it was put into the bath heated to 37 °C. We then checked CO2 levels in each flask every 2 minutes for 20 minutes. We came out results that showed a marginal difference between the amounts of CO2 produced at different temperatures. The results showed that increased temperature causes an increase in fermentation rate and increased production of CO2. Introduction: Fermentation is the break down of organic matter, by microorganism, in the absence of oxygen also known as anaerobic (Van Neil, 2008). Our reactions occurs when yeasts is added to a solution of glucose and water. Fermentation starts with a process called glycolysis. In glycolysis Glucose is broken down into two molecules of pyruvate and a net yield of 2 NADH (electron carrier) and 2 ATP (adenosine triphosphate) molecules. The first step of glycolysis is the energy investment phase. In which 2 ATP’s are added to the Glucose molecule, which produces 2 ADP’s and Fructose 1, 6-biphosphate. This is followed by the energy payoff phase. In this phase NAD+ is reduced to NADH and ADP is reduced to ATP. The total number of ATP created is 4 and 2 NAHDH. After the energy payoff phase what is left is 2 pyruvates. Fermentation then takes place only in the absence of oxygen. In fermentation the pyruvate is converted into ethyl alcohol, through the oxidation of the 2 NADH molecules, which returns them to two NAD+’s (Freeman, 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested yeasts ability to break down disaccharides, sugar in that case, at different temperatures and found that 37 °C was the optimal temperature for yeast to break down sugar, to formulate our hypothesis. Our sources we collected also indicated that different yeasts have different optimal operating temperatures, such as baker’s yeast, which requires higher temperature for yeast to ferment the proteins (Fell, 2008). Since we were using bakers yeast in our experiment we therefore came to the conclusion that increased temperature would increase yeasts ability to ferment glucose. Using this information and our sources we came up with the hypothesis that increasing the temperature of the solution would increase the rate of fermentation. We thought this was a reasonable hypothesis based upon earlier results from our other lab on temperatures affect on the yeasts ability to break down disaccharides. The predictions we came up with for the results of our tests were that the flasks at 37 °C would have a much more accelerated rate of CO2 production then that of the 25 °C Flasks. Materials and Methods: In the experiment we obtained 9 small beakers and 6 fermentation flasks. In the one beaker we added 18mL of Glucose. In the next we added 6ml of Yeast Suspension followed by another beaker with 18ml of distilled water. We then took those 3 beakers and placed them in the incubating bath set at 37ËšC for 5 minutes. After 5 minutes took the beakers out and added 6mL of distilled water, 2mL of yeast suspension and 6mL of Glucose into 3 separate beakers and mixed them together. We then immediately added them at the same time to separate fermentation flasks and measured their CO2 levels using a ruler. We then placed them in the incubating bath set for 37ËšC and set out timer for 2 minutes. We then prepared 3 beakers using 6mL of distilled water, 2mL yeast suspension and 6mL Glucose solution. Except that this time the yeast, water and glucose was a room temperature (25ËšC). We then proceeded to pour these mixtures into 3 separate fermentation flasks and measured their CO2 levels using a ruler. We then set a timer for 2 minutes. Each time the timer went off we would check the CO2 levels using a ruler. We continued to repeat this checking every 2 minutes for 20 minutes for each set of flasks. Results: My results indicated that increased temperature increased the rate of fermentation. In the CO2 Evolution graphs it is clear that as time increased as 2-4 minutes you can see a noticeable increase in the level of CO2 in the fermentation flask. As time increases that difference only increases and increases. Then when you look and the average alcohol fermentation graph it is clear that in total amount of CO2 produced in the flasks fermented in the 37ËšC incubating bath were much quicker in the process of fermentation, so therefore they produced much more CO2 then those at room temperature (25ËšC). Discussion: My Data supported my hypothesis. Each of my graphs data supported this finding. In the graph showing CO2 evolution the data showing 37ËšC had a steep positive slope, while the 25ËšC data showed an almost unnoticeable positive slope. This shows how over time the fermentation in the flasks at 37ËšC had a noticeable increase in its rate. The other graph shows the overall production of CO2 for each set of flasks. For the flasks at 25ËšC their average CO2 produced was .7mm, while the flasks at 37ËšC produced on average was 9.2mm. This increase rate and total production increase from that at 25ËšC and 37ËšC without a doubt supported my hypothesis. Also our minimization of errors landed itself to accurate results. We minimized any error by having the same person measure levels of CO2 and measure out substances such as yeast suspension. This increases my confidence that the results of our experiment not only support my hypothesis, but also supports that our bodies’ temperature (37ËšC) is the optimal temperature for cell respiration and not room temperature. Another follow-up experiment that could be used to give more detailed information about what happened is an experiment in which you run the same test, except include a 3rd condition in which the temperature is below room temperature such as 0ËšC. This could show the increase from freezing to room temperature and room temperature to 37ËšC. References: Cornelias B Van Niel, â€Å"Fermentation,† in AccessScience,  ©McGraw-Hill Companies, 2008. Web. Freeman, Scott. Biological Science. 4th ed. Boston: Benjamin Cummings, 2011. Print. Jack W. Fell, Herman J Phaff, Graeme M. Walker, â€Å"Yeast,† in AccessScience,  ©McGraw-Hill Companies, 2008. Web. Reddy. Effect of Fermentation Condition on Yeast Growth and Volatile Composition of Wine Produced from Mango Fruit Juice. Food Biproducts Processing: Transactions of the Institute of Chemical Engineers Part C 89.4 (2011): 487-91. EBSCO. Web. 2 Oct. 2012. Web.

Greek Architecture Essay -- Greece Essays Temples Building History

Greek Architecture The Greek culture has had a huge impact on the history of the world. There is something Greek in almost everything, especially in the world’s architecture. Greece no longer had one king, so they focused on building temples for their gods. Architecture began small and plain but evolved into impressive pieces of art. As time passed from the Archaic period to the Hellenistic period, the people of Greece developed a type of formula for their buildings and their pieces of art.   Ã‚  Ã‚  Ã‚  Ã‚  In Ancient Greece, religion dominated their life, so it is understandable that their architecture would be dominated by their religion. Before Greece became many different city-states, they had kings, and they would build a room called a megaron to show they King’s authority. A typical megaron is a single chamber with a fire pit in the middle with a throne off to the side. This room opened up into a porch (portico) that had four columns in the front. After there were no more kings these megarons turned into temples. The fire pit, which turned into some type of altar or a place to show a sacrifice for that god, was now outside in front of the temple. The altar was now outside so people could see you make these sacrifices to their gods. The first Greek temples were made out of wood and were long rectangular buildings with a porch all the way around which was supported by columns made from tree trunks. A Greek temple consists of five basic parts: the pediment, entablature, columns, base, and the cella, or the inner sanctum. The pediment is triangular and would have a type of sculpture or ornament known as acreteria on each corner. An entablature consists of three parts: the cornice, frieze, and architrave, which holds up the pediment. The columns are the support between the entablature and the base. The base usually consists of three steps. The cella is the inner sanctum, which has different rooms with more columns for support. Each temple would have a cella but they were not identical.   Ã‚  Ã‚  Ã‚  Ã‚  The Greeks developed an architectural system called an order, to distinguish the different styles of temples. Each order has a different proportions, entablature, and columns. The three Greek orders are called Doric, Ionic, and Corinthian. The Doric order is considered to be the masculine order. The columns are short and stout and very plain. A Doric column has no base a... ...he most important part of the agora. The stoa would be used for political, economical, or financial reasons. The Greeks also built gyms to exercise. During the Hellenistic period, the Greeks seemed to build more buildings where men could gather and socialize than places for worship.   Ã‚  Ã‚  Ã‚  Ã‚  During the Hellenistic period, you find the first monument built to celebrate someone who was not a general, god, or ruler. The Monument to Iysicrates is located in Athens, and is the first time a Corthinian column is used on the exterior. There was not a lot of money to spend on new temples but they still managed to build the Temple of Zeus Olympius. There is not much left of it today but it was huge. This temple was decastyle, meaning ten columns across, and the columns were sixty-five feet tall.   Ã‚  Ã‚  Ã‚  Ã‚  Even today the ideas and formulas of Greek architecture influence present day architects. When you think of Roman architecture, you think of the arch but even then they used the Imperial Motif to decorate their buildings. The Imperial Motif is the art of trabeation on arcuated architecture. The architecture of Ancient Greece will continue to influence the architecture of today and tomorrow.

The Story of Tom Brennan Quotes

Story of Tom Brennan quotes: 19 -â€Å"Sudden death, sudden death fellas. † 26 –â€Å"that was the thing I couldn’t get my head around – there would be a tomorrow, and a day after that, and a day after that. The world went on regardless of how I felt. † 71 –â€Å"All we could do was stand around†¦ Watching Finn and a machine that heaved and clicked with each breath it took for him. † 101- â€Å"I changed my clothes cause Finn’s blood was on my jeans and jumper, but I can still smell him on my skin, especially my hands though I was washed them a hundred times. 116 –â€Å"That’s all right, she whispered. How about you saying grace tonight? † 120 –â€Å"And if you’d asked me then what I thought, I would’ve said nothing or no one could take that away from us. † 124 –â€Å"like I said, that was a low point. † 128 –â€Å"Being a team player, you should have k nown better. † 132 –â€Å"Give him my love. She whispered. Tell him I’m always thinking of him. † 134 –â€Å"Didn’t they realise we weren’t like everyone else here? † 139 –â€Å"Tom, he swallowed. I’m sorry. I’m so sorry you had to leave the Billi. I’m so, so sorry. † 148 –â€Å"You could be Finn. 152 –â€Å"Talking about a waste. † 154 –â€Å"Fair? Kath yelled. Fair! † 157 –â€Å"One small step for man. One giant leap for mankind. Cause today, for my family, that’s what it was like. † 167 –â€Å"You know, today I’m going to the Hill Deli to buy one of their home-made lasagnes. I hear they’re delicious. † 183 –â€Å"When I walked into the school gates†¦ no one turned to look at us. No one pointed, no one whispered behind their hands. † 185 –â€Å"I felt lighter. I no longer had the bu rden of carrying a secret. And the consequences – well there was none. 185 –â€Å"At least she liked watching the footy show. We’d have a good laugh over that. Sometimes she wasn’t too bad. † 210 –â€Å"†I know it hurts Tom†¦ but we can’t undo what’s done. Bennie’s is giving you a chance and, as pathetic as it may seem to you, it’s still a chance. † 217 –â€Å"We’d reached the top of the ascent. I’d made it up without even realising. † 260 –â€Å"Blue upon blue which was what our families had become – sad, angry, guilt ridden, confused, lost. Blue or for some of us black. † 283 –â€Å"That was the morning Tom Brennan came back forever. †

Symptoms And Symptoms Of Anxiety Essay - 1822 Words

40 million people over the age of 18 in the United States suffer from anxiety. My sister Samantha is one of 40 million (Elements Behavioral Health, n.d.). She has just recently been subscribed medication to help subdue the severity of her illness. What is anxiety? According to ADDA Generalized Anxiety Disorder is characterized by persistent, excessive, and unrealistic worry about everyday things (Anxiety and Depression Association of America, n.d.). Samantha is 23 years old and is someone who at one point in my life I did not look up to. Now, she is someone I would consider a role model. There is a 4-year age difference between Sam and I. We were at different stages in our lives and that made it very difficult of us to get along. I judged everything that she did in her life. It wasn’t until a few years ago that I became open minded and realized that growing up isn’t easy. She chose not go to college and moved out when she was 20. Samantha has a lot of offer this world b ut has made it clear that it is not easy growing up and she is still trying to figure it out. One of the main reasons that Samantha is having a difficult time figuring out what she wants to do with her life is because her anxiety gets the best of her. As she becomes more aware of her illness she is trying to overcome it and be the best version of herself that she can. During our interview I learned a lot of new information about my sister that I did not know before. We started off our interview by going overShow MoreRelatedSymptoms And Symptoms Of Anxiety1008 Words   |  5 PagesAnxiety Anxiety, depression, and medications†¦ These are all things we need to take into consideration when thinking about everyday life. Anxiety has become a generalized factor of life and many people suffer from it everyday. Yes, everyone knows what it s like to feel anxious but not like those with anxiety. 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